RNA

Part:BBa_K3805999

Designed by: Yumo Li   Group: iGEM21_BNU-China   (2021-09-30)
Revision as of 12:29, 20 October 2021 by TheThirdSanta (Talk | contribs)


sgRNA bind to T7 promoter


We designed the sgRNA (19 bp) that can bind to the T7 promoter, which can guide the dCas9 to bind to the T7 promoter and inhibit the function of the promoter.

How does it work?

Compared with traditional Cas9, dCas9 does not have the function of knockout, but only retains the ability to be guided into the genome by sgRNA. dCas9 can fusion or recruit transcription factors. Through the guide of sgRNA which can combine with T7 promoter specifically, dCas9 can accurately combine with the target region and regulate downstream gene transcription. The combination have the effect of inhibition of targeted gene transcription. This process can silence gene expression in cells effectively.

DCas9.jpeg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None