Coding

Part:BBa_K4015002

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-19)
Revision as of 11:30, 20 October 2021 by Anrdew (Talk | contribs)

DsbA

DsbA is a soluble periplasmic protein. It belongs to the thioredoxin family and acts as a type of signal peptide which serve as the primary source of disulfide bonds to secreted proteins. The protein catalyzes the oxidation of the two cysteine sulfhydryls and form a covalent S-S bond connecting amino acid, then donates to the secreted protein, catalyzing their oxidation and their folding. DsbA’s size is about 21-kDa, primarily active in type II secretion peptide.

Usage and Biology

In our project, we pick three signal peptides to help secrete our target proteins, which are HlyA、GeneIII and DsbA . We used p47 vector containing GFP to compare the effectiveness of these signal peptides. p47-GFP-HlyA displayed a lower level of fluorescence comparing to p47-GeneIII-GFP, suggesting HlyA is more effective in secretion since more GFP has entered the medium. p47-DsbA-GFP showed no fluorescence, meaning protein expression was inhibited or the folding of the protein has failed. 

T--KEYSTONE--GFP.png

 Fig. Result of GFP secretion experiment 


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None