Coding

Part:BBa_K3989014

Designed by: Song-Yuan Zhang   Group: iGEM21_UZurich   (2021-10-01)
Revision as of 10:35, 20 October 2021 by ZhangSongyuan (Talk | contribs)


double cellulose binding domain fused with ClyA for OMV display

In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display the double cellulose-binding domain (dCBD, Part:BBa_K1321340) to the surface of bacterial outer membrane vesicles (OMVs). To make dCBD better displayed, we put multiple linkers and a 3×FLAG tag between ClyA and dCBD to minimize the steric hindrance.

Usage and Biology

Chracterization by team 2021 UZurich

Seedling growth inhibition assay was performed to test whether displaying cellulose-binding domain on the surface can enhance the plant immune response triggered by OMVs.

ClyA-dCBD-SGI.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None