Composite

Part:BBa_K4015007

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-19)
Revision as of 10:28, 20 October 2021 by Anrdew (Talk | contribs)


CotA-Lcp-HlyA

CotA-Lcp-HlyA is composed of three basic parts, Lcp1VH2, HlyA and CotA. HlyA and Lcp are connected by Gibson assembly in order to allow Lcp to be secreted outside the cell through HlyA,and assembling CotA on the composite part can help to indicate the secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 286


[edit]
Categories
Parameters
None