Coding

Part:BBa_K4015001

Designed by: Yu Jun   Group: iGEM21_KEYSTONE   (2021-10-19)
Revision as of 10:14, 20 October 2021 by Anrdew (Talk | contribs)


Lcp1VH2

The Lcp1VH2 is a strain of latex clearing protein extracted from Gordonia polyisoprenivorans VH2, a species of Gordoniaceae, which is of interest because of its ability to damage natural rubber. Latex clearing protein was first described by Rose (Rose et al., 2005) and the synthesis of Lcp1VH2 into e.coli was first described by Hiessl et. al (Hiessl et al., 2014). The Lcp1VH2 belongs to oxygenate, which can catalyse the extracellular cleavage of poly (cis-1,4-isoprene) through adding two oxygen molecules to the chemical bond where the polyisoprene is attached(Ilcu et al., 2017). The molecular mass of latex clearing protein is 42 kDa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 112
    Illegal SapI.rc site found at 100


[edit]
Categories
Parameters
None