Coding

Part:BBa_K3815004

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-15)
Revision as of 10:03, 20 October 2021 by Shiotsu (Talk | contribs)


NOP1-Mxe GryA intein-PT-linker-ELK16

Descriotion of this part

Targeted protein

This part is for the purfication of the small peptide, NOP1. It can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene that promotes the wilting of a plant and aging of cut flowers.

Purification system

In order to purify the peptide, we adopted ELK16 system that the past iGEM teams had not used. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 117
    Illegal NgoMIV site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None