Plasmid

Part:BBa_K4083000:Design

Designed by: Arsen Orazbek   Group: iGEM21_NU_Kazakhstan   (2021-09-08)
Revision as of 09:59, 20 October 2021 by Arsenorazbek (Talk | contribs) (→‎Design Notes)

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pRGPDuo2 plasmid for P. putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3853
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3880
    Illegal BamHI site found at 31
    Illegal XhoI site found at 2175
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6480
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

Modified from pRG_Duet for Corynebacterium glutamicum Gauttam et al.

References

[1] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514