Part:BBa_K4083007
rhlA gene with SalI and SacI sites at ends
rhlA gene codes for rhamnosyltransferase chain A (RhlA) in Pseudomonas aeruginosa.
Usage and Biology
The P. aeruginosa is gram-negative bacillus, and opportunstic pathogen. It secretes rhamnolipids - the rhamnose containing glycolipid biosurfactant. This biosurfactants are used by P. aeruginosa to emulsify the oil sunstances for easy digestion. Thus, rhamnolipids can increase the availability of fats wchich can be important in many different areas like petrleum, bioremediation, cosmetics, food, agroculture, etc. [1] However, due to the toxicity and infectiousness of P. aeruginosa, other alternative organisms are tested. Currently, he organism that showed the best results is Pseudomonas putida.
Our team planned to extract rhL and rhlB genes from P.aeruginosa and to insert them into pRGPDuo2 plasmid obtained from Gauttam, R. [2] We developed the new approach to increase the P. putida's rhamnolpid synthesis by adding nadE gene which encodes NAD synthetase. This way, we hoped to see more rhamnolipid production in engineered P. putida.
The rhamnosyltransferase chain A catalyzes the production of 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) from β-hydroxydecanoyl-ACP. HAA is the precursor for rhanolipid production which is catalyzed by RhlB to form mono-rhamnolipids [1].
Reference:
[1] Chong, H., & Li, Q. (2017, August 5). Microbial production of rhamnolipids: opportunities, challenges and strategies. Microbial Cell Factories. https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0753-2
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 94
Illegal BamHI site found at 654
Illegal XhoI site found at 830 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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