Coding

Part:BBa_K3815002

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-15)
Revision as of 09:41, 20 October 2021 by Shiotsu (Talk | contribs)


Defensin1-Mxe GryA intein-PTlinker-ELK16

Descriotion of this part

Targeted protein

This part is for the purfication of antimicrobial peptide, Defensin1. This is derived from Homo Sapiens. This can inhibit the growth of the gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Purification system

In order to purify the peptide, we adopted ELK16 system that the past iGEM teams had not used. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None