Coding
a-galRFP

Part:BBa_K4032005

Designed by: Tsugumi Ohzeki   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 09:02, 20 October 2021 by Tsugumi (Talk | contribs)


α-galactosidase-RFP

This part contains the gene of the α-galactosidase-RFP fusion protein.


Usage and Biology

This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. https://www.uniprot.org/uniprot/P06720

Red fluorescence of RFP is observed under the fluorescence microscope and natural light.


Designs

This part expresses the α-galactosidase-RFP fusion protein.

α-galactosidase is from E.coli K-12 MG1655 and RFP is from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .

We removed the stop codon of alpha-galactosidase and the start codon of RFP and connected the ends.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1905
    Illegal AgeI site found at 2017
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None