Plasmid

Part:BBa_K3887004

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)
Revision as of 08:40, 20 October 2021 by Ruijuan Xiang (Talk | contribs)


pCas

pCas9 is a plamid containing Cas9 editing enzyme, used for gene modification at a specific location. Cas9 protein follows guide RNA to cut the two strands of DNA at a designated location, where strands of DNA can be edit (insertion, deletion,mutation). In our project，we used this plasmid to knockout TnaA on E. coli BL21(DE3) genome. Below is the map of it.

T--BJU China--Fig.1 pCas9-1.png

Figure.1 Snapgene map of pCas9

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6015
    Illegal NotI site found at 3034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8294
    Illegal BamHI site found at 10296
    Illegal XhoI site found at 4353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10131
    Illegal AgeI site found at 11788
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 10113


[edit]
Categories
Parameters
None