Coding

Part:BBa_K2333011

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-10-27)
Revision as of 07:25, 20 October 2021 by AshWinShaRma (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


mf-Lon

This part codes for the AAA+ Lon Protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. The orthogonal nature of this protease was notably used by Collins et al. 2014 ("Tunable Protein Degradation in Bacteria"), where a series of mf-Lon associated protein degradation tags were designed via mutagenesis to have distinct levels of affinity for the protease and therefore distinct associated degradation rates. These tags are available on the registry in the range BBa_K2333001-Bba_K2333006.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1834
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 2124
    Illegal AgeI site found at 2149
  • 1000
    COMPATIBLE WITH RFC[1000]


Modifications in Sequence : iGEM21_IISER-Tirupati_India

BBa_K3889020 is a modified version specific to our project's assembly. Mutated multiple RE sites to make it assembly compatible.

[edit]
Categories
Parameters
None