Coding
Part:BBa_K3799027
Designed by: Shubhamay Das, Tanya Ivanov, Franklin Zheng Group: iGEM21_IISER_Kolkata (2021-10-19)
Type III-A CRISPR-Cas effector ribonuclease - Csm6 with Histag and TEV cut site
Csm6 is a non-specific endoribonuclease that cleaves single-stranded RNA upon activation by cyclic adenylates or linear adenine homopolymers terminated with a 2'3'-cyclic phosphate.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 501
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 461
Illegal BglII site found at 1403 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |