Composite

Part:BBa_M10218

Designed by: Jennifer Brophy   Group: Berkeley BioE140L - S09   (2009-05-10)
Revision as of 04:32, 11 May 2009 by Registry (Talk | contribs)

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{Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPG_L6!}{dblTerm}

Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a leucine zipper peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
    Illegal AgeI site found at 2228
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005


[edit]
Categories
Parameters
None