Coding

Part:BBa_M10052:Design

Designed by: Susan Chen   Group: Berkeley BioE140L - S09   (2009-05-06)
Revision as of 03:01, 11 May 2009 by Susanychen (Talk | contribs) (→‎Source)

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{˂ehaB!}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 589
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 589
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 589
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 589
    Illegal AgeI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A

Source

Genome of Enterohemorrhagic Escherichia Coli O157:H7 EDL933 

Construction of ehaB Basic Part 
PCR Osc0030F/Osc0031R on ehaB gene           (1257bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5               (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10052  {<ehaB!]
-------------------------------------
Osc0030F  Forward Biobricking of ehaB  
ggcctGAATTCattAGATCTggcttaatcgcaatgaactct
Osc0031R  Reverse Biobricking of ehaB
gtaccGGATCCttaccaggtatatttaacac

References

Wells, Timothy J., Sherlock, Orla, Rivas, Lucy, Mahajan, Arvind, Beatson, Scott A., Torpdahl, Mia, Webb, Richard I., Allsopp, Luke P., Gobius, Kari S., Gally, David L., and Mark A. Schembri1 (2008) EhaA is a novel autotransporter protein of enterohemorrhagic Escherichia coli O157:H7 that contributes to adhesion and biofilm formation. Environmental Microbiology 10: 589-604.

Wells, Timothy J., McNeilly, Tom N., Totsika, Makrina, Mahajan, Arvind, Gally, David L., and Mark A. Schembri. (2009) The Escherichia coli O157:H7 EhaB autotransporter protein binds to laminin and collagen I and induces a serum IgA response in O157:H7 challenged cattle. Environmental Microbiology.