Coding

Part:BBa_K3738020:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-19)
Revision as of 02:14, 20 October 2021 by Registry (Talk | contribs)

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Lbu-Cas13a with an N-Terminal 6xHistidine Tag and C-Terminal Anionic Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1089
    Illegal PstI site found at 1944
    Illegal PstI site found at 2883
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1089
    Illegal PstI site found at 1944
    Illegal PstI site found at 2883
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 370
    Illegal BglII site found at 1306
    Illegal BglII site found at 1516
    Illegal BglII site found at 1570
    Illegal BglII site found at 1801
    Illegal BglII site found at 2074
    Illegal BglII site found at 2560
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1089
    Illegal PstI site found at 1944
    Illegal PstI site found at 2883
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1089
    Illegal PstI site found at 1944
    Illegal PstI site found at 2883
    Illegal NgoMIV site found at 2022
    Illegal NgoMIV site found at 2658
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The anionic tag is present to facilitate uptake into our delivery particle MS2 whereas the Histidine tag was added for the purpose of Nickel-Affinity chromatography in obtaining the purified protein.


Source

The Cas13a protein comes from the Gram-negative bacteria Leptotrichia buccalis (Lbu).

References