Part:BBa_K3726066

Lv.1 HspA - A

This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726061” L0_HspA* which codifies the major heat shock protein capable of enhancing cellular fitness in the presence of environmental stresses such as solvent presence.

Within our project we have used HspA to reduce ROS stress generated from n-butanol.

The part in its upstream region has a homology region “BBa_K3726108” 5CON1(H)_NS3(mod)-up (PCC 11801) for homologous recombination within the genome of PCC 11801. In the downstream region, the part has the connector 3'Con1 “BBa_K2560070” that will allow the assembly of a Lv2 construct following the marburg collection standard.

Likewise, a chloramphenicol resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the strong constitutive promoter P_PpsbA*-RiboJ “BBa_K3726079” and the strong synthetic RBS, RBS_PtaBs “BBa_K3726092”

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1139
  Illegal PstI site found at 798
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1139
  Illegal PstI site found at 798
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1139
  Illegal XhoI site found at 2860
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1139
  Illegal PstI site found at 798
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1139
  Illegal PstI site found at 798
  Illegal NgoMIV site found at 724
  
• 1000
  COMPATIBLE WITH RFC[1000]