Composite

Part:BBa_K3726055:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)
Revision as of 01:10, 20 October 2021 by Sbarraga (Talk | contribs)

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L1_MCGII-A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal NheI site found at 5831
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal BglII site found at 1234
    Illegal BglII site found at 3274
    Illegal BglII site found at 4638
    Illegal BamHI site found at 3372
    Illegal BamHI site found at 3387
    Illegal BamHI site found at 7086
    Illegal BamHI site found at 7544
    Illegal BamHI site found at 8517
    Illegal XhoI site found at 337
    Illegal XhoI site found at 2064
    Illegal XhoI site found at 2709
    Illegal XhoI site found at 3183
    Illegal XhoI site found at 5713
    Illegal XhoI site found at 6380
    Illegal XhoI site found at 6513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2292
    Illegal EcoRI site found at 2733
    Illegal EcoRI site found at 4492
    Illegal EcoRI site found at 5608
    Illegal EcoRI site found at 5692
    Illegal EcoRI site found at 8747
    Illegal PstI site found at 1089
    Illegal PstI site found at 2877
    Illegal PstI site found at 2976
    Illegal PstI site found at 3561
    Illegal PstI site found at 4519
    Illegal PstI site found at 4747
    Illegal PstI site found at 6257
    Illegal NgoMIV site found at 2820
    Illegal NgoMIV site found at 4209
    Illegal NgoMIV site found at 4383
    Illegal NgoMIV site found at 4533
    Illegal NgoMIV site found at 7707
    Illegal AgeI site found at 4834
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the parts “BBa_K3726110” 5CON1(H)_nNS4-up (PCC 11801) and “BBa_K3726111” 3CON5(H)_nNS4-down (PCC 11801) allowing the Integration within the PCC 11801 Genome.

Source

This construct has been made by golden gate reaction.

References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.