![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K3792006
Designed by: Chase Tarillo Group: iGEM21_FSU (2021-09-24)
Revision as of 00:47, 20 October 2021 by Anishshahez (Talk | contribs)
NodCIJ Generator I
The NodCIJ Generator with Weak Promoter does not produce a lot of the NodC protein, NodI protein, and NodJ protein. The NodC protein produces chitin and NodI and NodJ transports nodulation factors.
This part consists of 2555 base-pair length. It includes a promoter, 3 ribosome binding sites, 3 protein-coding sequences, and a terminator. We used promoter K2832184, a weak promoter from the BIOFAB collection. In addition, we also used protein-coding sequences from Chitin synthase from Rhiozobium leguminosarum, Nodl a protein possibly involved in transporting chitin across the cell membrane, and NodJ, a protein also possibly involved in the transportation of chitin
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 492
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 854
[edit]
Categories
Parameters
None |