Composite

Part:BBa_K3886010

Designed by: Meng Fankang   Group: iGEM21_NDNF_China   (2021-10-01)
Revision as of 00:24, 20 October 2021 by MengFankang Nankai (Talk | contribs)


Homologous-Barcode-pBAD-Kid-Homologous

Homologous sequence attached to the 5' and 3' end of Trace and Control System (Figure 1A) were used to integrated to the E. coli fimA site using CRISPR-Cas9 system (Figure 1B) by NDNF_China iGEM 2021 team. More information about Trace and Control System, please visit part: BBa_K3886022.

<img src="T--NDNF_China--part_traceandcontrol_genomeediting.png" alt="" width="700">

Figure 1: (A)The design scheme of Trace and Control System in Hidro; (B) The gel image of the result of genome integration of Trace and Control System into fimA site.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2030
    Illegal AgeI site found at 1613
    Illegal AgeI site found at 2302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1595


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Categories
Parameters
None