Coding

Part:BBa_K4080006

Designed by: Dessy Widyasari, Sania Isma Yanti   Group: iGEM21_UBrawijaya   (2021-10-19)
Revision as of 23:30, 19 October 2021 by Registry (Talk | contribs)

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eCPX (Enhanced Circularly Permuted Outer Membrane Protein X)

Enhanced CPX (eCPX) is a circularly permuted form of the OmpX protein that has been optimized to display unconstrained (15mer) peptides in E. coli [1]. eCPX is an outer membrane display scaffold that yields a more robust display scaffold to present diverse peptides as C- and N-termini fusions on the surface of bacteria. eCPX has a display rate that substantially improves relative to CPX and OmpX. This part can be used to improve the display of diverse peptides by reducing the impact of the passenger peptide upon the surface localization efficiency [2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None