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Coding
Part:BBa_K4080006
Designed by: Dessy Widyasari, Sania Isma Yanti Group: iGEM21_UBrawijaya (2021-10-19)
eCPX (Enhanced Circularly Permuted Outer Membrane Protein X)
Enhanced CPX (eCPX) is a circularly permuted form of the OmpX protein that has been optimized to display unconstrained (15mer) peptides in E. coli [1]. eCPX is an outer membrane display scaffold that yields a more robust display scaffold to present diverse peptides as C- and N-termini fusions on the surface of bacteria. eCPX has a display rate that substantially improves relative to CPX and OmpX. This part can be used to improve the display of diverse peptides by reducing the impact of the passenger peptide upon the surface localization efficiency [2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |