Composite

Part:BBa_K3726046

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)
Revision as of 22:37, 19 October 2021 by Sbarraga (Talk | contribs)

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BOH1_C_Lv1

This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726014” CDS_Lv0_BOH_1_C , the last two enzymes of synthetic butanol biosynthesis operon.

The part in its upstream region, has the connector 5'Con3 “K2560067” that will allow the assembly of a Lv2 construct following the marburg collection standard. In its downstream region has a homology region “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) for homologous recombination within the genome of PCC 11801.

Likewise, a Kanamycin resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the medium-strong synthetic constitutive promoter J23119 “BBa_K2560031”and by the synthetic RBS, RBS* “BBa_K3726093”, which has demonstrated its increased ribosome binding strength in Synechocystis PCC6803 and other cyanobacteria in comparison with other heterotroph-derived RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1723
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 431
    Illegal NheI site found at 454
    Illegal NheI site found at 729
    Illegal NheI site found at 957
    Illegal PstI site found at 1723
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2057
    Illegal BglII site found at 2174
    Illegal BglII site found at 4593
    Illegal XhoI site found at 3277
    Illegal XhoI site found at 4120
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1723
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1723
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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