Part:BBa_K3886005
NDNFCHINAiGEM2021 Barcode
NDNFCHINA2021 Barcode
BIOT is a basic design for the expression of Geobacter sulfurreducens's pilin-based electrically conductive protein nanowires (e-PNs)[1]. The system includes a tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1A). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.
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Characterization
In order to improve the protein nanowire, we mutated the RBSs of the TypeIV pilin assembly system and the pilA gene to enhance the productivity and output voltage, respectively.
Sequence and Features| None |