Composite

Part:BBa_K3889110

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-08)
Revision as of 22:25, 19 October 2021 by AshWinShaRma (Talk | contribs)


XylR Repressor Cassette for Bacillus subtilis

This gene cassette has been designed to be a part of the genetic circuit for an inducible kill switch in Bacillus subtilis. The gene coding for the XylR repressor ( protein is downstream to the P43 constitutive promoter of Bacillus subtilis (BBa_K143013) and RBS for Bacillus subtilis. The xylR gene is upstream to porcine teschovirus-1 P2A Peptide Linker for effective transcription. Downstream to the linker, a reporter gene sfGFP (BBa_K3889002) has been placed to check the working of the cassette in Bacillus subtilis. The cassette is complete with the double terminator (BBa_B0015).

The cassette has been designed to be a part of a genetic circuit of a xylose inducible kill switch. The constitutive expression of the XylR repressor will suppress the production of the toxin in absence of xylose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1196


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Categories
Parameters
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