Composite

Part:BBa_K3726044

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-19)
Revision as of 22:01, 19 October 2021 by Sbarraga (Talk | contribs)

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BOH_1_A_LV1

This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726012” CDS_Lv0_BOH_1_A , the first and the second two enzymes of synthetic butanol biosynthesis operon.

The part in its upstream region has a homology region “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) for homologous recombination within the genome of PCC 11801. In downstream region, the part has the connector 3'Con1 “BBa_K2560070” that will allow the assembly of a Lv2 construct following the marburg collection standard

Expression is controlled by the strong constitutive promoter P_PpsbA*-RiboJ “BBa_K3726079” and by the synthetic RBS, RBS* “BBa_K3726093”, which has demonstrated its increased ribosome binding strength in Synechocystis PCC6803 and other cyanobacteria in comparison with other heterotroph-derived RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1230
    Illegal EcoRI site found at 1850
    Illegal PstI site found at 18
    Illegal PstI site found at 1755
    Illegal PstI site found at 2003
    Illegal NgoMIV site found at 1494
    Illegal NgoMIV site found at 1594
    Illegal NgoMIV site found at 1900
    Illegal AgeI site found at 594
    Illegal AgeI site found at 2272
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None