Part:BBa_M10024

{a~Int-native>} Intimin (native)

This part is derived from the eaeA gene found in enterohemorrhagic Escherichia coli O157:H7. The eaeA gene is responsible for intimin, which are bacterial adhesins that allow for interaction between E. coli and a range of eukaryotic cell surface receptors. The intimin has a N-terminal transporter domain that is localized to the outer membrane. The transporter promotes the translocation of C-terminally attached passenger domains accross the membrane and displays it on the cell surface.

This part consists of the N-terminal transporter with a prepro sequence. Passengers of interest can be fused onto the C-terminal of the part to display the passenger on the cell surface.
PMID: 11717287

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This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]
Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1248