Composite

Part:BBa_K4046810:Design

Designed by: Jessica Shah   Group: iGEM21_Duke   (2021-10-19)
Revision as of 20:48, 19 October 2021 by Shahjessica (Talk | contribs)


CMV - BS #1 - BS #1 - Kozak - cLuc - bghA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
    Illegal BglII site found at 1278
    Illegal BglII site found at 1337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene was synthesized commercially from the published sequence (IDT). cLuc is an enzyme isolated from the genomic sequence of V. hilgendorfii, a marine crustacean. This gene was obtained through PCR of a commercially available plasmid pIND-CLucZ (Addgene, 53224).



Source

This is an identified binding site for the DhdR gene, and is generated from sequence analysis.


References

A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR Dan Xiao, Wen Zhang, Xiaoting Guo, Yidong Liu, Chunxia Hu, Shiting Guo, Zhaoqi Kang, Xianzhi Xu, Cuiqing Ma, Chao Gao, Ping Xu bioRxiv 2021.02.18.430539; doi: https://doi.org/10.1101/2021.02.18.430539