Part:BBa_K4046770:Design

CMV - BS #2 with spacer - BS #2 - Kozak - tdTomato - bghA

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1025
  Illegal PstI site found at 1751
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1025
  Illegal PstI site found at 1751
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 614
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1025
  Illegal PstI site found at 1751
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1025
  Illegal PstI site found at 1751
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This gene was synthesized commercially from the published sequence (IDT). tdTomato is the modified product of a gene originally from mushroom corals in the Discosoma family. tdTomato is the modified product of a gene originally from mushroom corals in the Discosoma family. The gene was obtained through PCR of a commercially available plasmid pCRISPR-HOT_tdTomato (Addgene, 138567).

Source

This is an identified binding site for the DhdR gene, and is generated from sequence analysis.

References

A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR Dan Xiao, Wen Zhang, Xiaoting Guo, Yidong Liu, Chunxia Hu, Shiting Guo, Zhaoqi Kang, Xianzhi Xu, Cuiqing Ma, Chao Gao, Ping Xu bioRxiv 2021.02.18.430539; doi: https://doi.org/10.1101/2021.02.18.430539

Sidney Cambridge (ed.), Photoswitching Proteins: Methods and Protocols, Methods in Molecular Biology, vol. 1148, DOI 10.1007/978-1-4939-0470-9_7, © Springer Science+Business Media New York 2014