Composite

Part:BBa_K4046730

Designed by: Andy Qiao   Group: iGEM21_Duke   (2021-10-19)
Revision as of 20:32, 19 October 2021 by Registry (Talk | contribs)

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CMV - BS #1 - BS #1 - BS #1 - Kozak - tdTomato - bghA

This is a binding site for the DhdR gene, as identified in the literature. In this composite part, we added a Kozak sequence and tdTomato gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1040
    Illegal PstI site found at 1766
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None