Tag
MFa1-Secre

Part:BBa_K792002

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-24)
Revision as of 20:13, 19 October 2021 by CWChew (Talk | contribs)

Secretion tag from yeast α-factor mating pheromone (MFα1)

This part is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER.


To use this tag, attached it directly to your proteins CDS.


See also: A Kozak sequence from α-factor mating pheromone (MFα1) BBa_K792001
This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. Igem.bsas.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Part Contributions

NUS Singapore 2021

Authors:

Tania Santosh Nair, Chew Chin Wei, Linus Tan

Contribution Summary:

[[File:|700px|thumb|center|]]

Additional Information

Please visit the part registry page for our improved part No argument specified with partinfo tag. for more information on the part improvement.


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/localization
Parameters
None