Part:BBa_K1159105

Mature Nuclease NucA from Staphylococcus aureus (Thermonuclease) in RFC[25]

Thermonuclease is a endo-exonuclease from Staphylococcus aureus that degrades dsDNA, ssDNA, dsRNA and ssRNA. This part is coding the mature form of the nuclease, also called NucA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)  ATGGCCGGCGCAACTTCA ... TCAGGTCAAACCGGT  ORF from nucleotide position -8 to 453 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below) 1  101  MAGATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGK MVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQTG*
Sequence features: (with their position in the amino acid sequence, see the list of supported features) None of the supported features appeared in the sequence
Amino acid composition: Ala (A) 15 (9.7%) Arg (R) 5 (3.2%) Asn (N) 6 (3.9%) Asp (D) 8 (5.2%) Cys (C) 0 (0.0%) Gln (Q) 6 (3.9%) Glu (E) 12 (7.8%) Gly (G) 12 (7.8%) His (H) 4 (2.6%) Ile (I) 5 (3.2%) Leu (L) 11 (7.1%) Lys (K) 23 (14.9%) Met (M) 5 (3.2%) Phe (F) 3 (1.9%) Pro (P) 6 (3.9%) Ser (S) 5 (3.2%) Thr (T) 11 (7.1%) Trp (W) 1 (0.6%) Tyr (Y) 7 (4.5%) Val (V) 9 (5.8%)
Amino acid counting Total number: 154 Positively charged (Arg+Lys): 28 (18.2%) Negatively charged (Asp+Glu): 20 (13.0%) Aromatic (Phe+His+Try+Tyr): 15 (9.7%)	Biochemical parameters Atomic composition: C763H1231N213O230S5 Molecular mass [Da]: 17228.8 Theoretical pI: 9.48 Extinction coefficient at 280 nm [M-1 cm-1]: 15930 / 15930 (all Cys red/ox)
Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges
Codon usage Organism: E. coli B. subtilis S. cerevisiae A. thaliana P. patens Mammals Codon quality (CAI): good (0.79) excellent (0.83) excellent (0.82) excellent (0.81) good (0.72) acceptable (0.60)
Alignments (obtained from PredictProtein.org) SwissProt: P00644 (99% identity on 150 AAs), Q5HHM4 (98% identity on 150 AAs), Q6GB41 (98% identity on 150 AAs), Q6GIK1 (98% identity on 150 AAs), Q7A6P2 (98% identity on 150 AAs), Q8NXI6 (98% identity on 150 AAs), Q99VJ0 (98% identity on 150 AAs) TrEML: A3QSR2 (98% identity on 140 AAs), A3QSR3 (98% identity on 140 AAs), A3QSR6 (98% identity on 140 AAs), A5A513 (98% identity on 148 AAs), A5A520 (98% identity on 148 AAs), A5A523 (98% identity on 148 AAs), A5IQZ5 (98% identity on 150 AAs), A6QFA0 (98% identity on 150 AAs), A6TZS0 (98% identity on 150 AAs), A7WZZ3 (98% identity on 150 AAs) PDB: 1ey0 (100% identity on 136 AAs), 1eyd (100% identity on 136 AAs), 1nsn (100% identity on 138 AAs), 1snc (100% identity on 135 AAs), 1sta (100% identity on 135 AAs), 1stb (100% identity on 136 AAs), 1stg (100% identity on 136 AAs), 1sth (100% identity on 136 AAs), 1stn (100% identity on 136 AAs), 1sty (100% identity on 136 AAs)
Predictions (obtained from PredictProtein.org)
Subcellular Localization (reliability in brackets) Archaea: cytosol (100%) Bacteria: secreted (55%) Eukarya: cytosol (24%)	Gene Ontology (reliability in brackets) Molecular Function Ontology: - Biological Process Ontology: GO:0030541 (28%)
Predicted features: Disulfid bridges:  - Transmembrane helices:  -
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