Part:BBa_K4092012

Specificity control toehold for Fusarium oxysporum

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design. This toehold is functional at a temperature of 37°C as a negative control for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), since it has single nucleotide mutations which should not allow the expression of any reporter gene. It has a MFE of -26.70000076 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]