Part:BBa_K1323012

Spc - Spectinomycin Resistance Gene Coding Sequence

This coding sequence is for the antibiotic spectinomycin. The sequence of this gene is obtained from pSPC vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The 618 bp of the CDS was changed from T to A to eliminate illegal pst1 site. The working concentration of spectinomycin of 1000 µg/mL in S.aureus/S.epidermidis hosts (Bose et al., 2013).

Contribution from other teams

Toulouse_INSA-UPS 2021's contribution

Characterization

Toulouse_INSA_UPS_2021contributed to the characterization of this part. The spcR gene had not been characterized before in iGEM, but the team showed this year that it is functional for a transformant selection in S. elongatus cyanobacteria. Check the part of the full construction (BBa_K3930026) for more result details.

(--ThomasG 16:53, 18 October 2021 (UTC+2))

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 488
  

References

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.