Coding

Part:BBa_K3861022

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 17:23, 19 October 2021 by Martje1998 (Talk | contribs)


hilA transcriptional regulator

hilA, together with hilC and hilD, are global regulators and control the expression of the Salmonella pathogenicity island I (SPI-1) genes in S. typhimurium on which the SPI-1 T3SS injectisome is encoded 1. Over 25 genes that are needed for host invasion are located on the SPI-1 2. Also, hilA activates the SPI-1 operon mediated by the prg and inv promoters 3,4. Thus, hilA controls SPI genes in response to enviromental needs like pH and oxygen levels4–6.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1635
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
    Illegal NgoMIV site found at 151
    Illegal AgeI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Widmaier, D. M. et al. Engineering the Salmonella type III secretion system to export spider silk monomers. Mol. Syst. Biol. 5, 309 (2009).
2. Heran Darwin, K. & Miller, V. L. InvF Is Required for Expression of Genes Encoding Proteins Secreted by the SPI1 Type III Secretion Apparatus in Salmonella typhimurium. J. Bacteriol. 181, 4949–4954 (1999).
3. Bajaj, V., Hwang, C. & Lee, C. A. hilA is a novel ompR/toxR family member that activates the expression of Salmonella typhimurium invasion genes. Mol. Microbiol. 18, 715–727 (1995).
4. Bajaj, V., Lucas, R. L., Hwang, C. & Lee, C. A. Co-ordinate regulation of Salmonella typhimurium invasion genes by environmental and regulatory factors is mediated by control of hilA expression. Mol. Microbiol. 22, 703–714 (1996).
5. Ahmer, B. M., van Reeuwijk, J., Watson, P. R., Wallis, T. S. & Heffron, F. Salmonella SirA is a global regulator of genes mediating enteropathogenesis. Mol. Microbiol. 31, 971–982 (1999).
6. Main-Hester, K. L., Colpitts, K. M., Thomas, G. A., Fang, F. C. & Libby, S. J. Coordinate regulation of Salmonella pathogenicity island 1 (SPI1) and SPI4 in Salmonella enterica serovar Typhimurium. Infect. Immun. 76, 1024–1035 (2008).

[edit]
Categories
Parameters
None