Regulatory

Part:BBa_K3737004

Designed by: Ruby Lovasz   Group: iGEM21_Alma   (2021-10-19)
Revision as of 16:33, 19 October 2021 by Registry (Talk | contribs)

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TetR repressor via estrogen binding

This part is an improvement upon the K123002 composite part. The improved part has a J23100 promoter in place of the LacIQ promoter and includes a ribosome binding site (RBS). The ssrA TetR degradation tag at the end of the DNA sequence was weakened.

This part is designed to be used with Estrogen Receptor (ER) because of its Human Estrogen Response Element (HERE). The ER binds the HERE and prevents TetR from getting promoted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None