Composite

Part:BBa_K3877005

Designed by: Yang Yu   Group: iGEM21_DKU   (2021-10-19)
Revision as of 14:39, 19 October 2021 by Registry (Talk | contribs)

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Tandem Algicidal Peptide SUMO His Tag Expression

Algicidal peptide coding gene after the codon optimization. The products are algicidal peptides that can be used as a highly efficient and targeted measure to terminate urgent ongoing cyanoHAB. The synthesized fragment contains (1) His-tag at the beginning for future purification, (2) sumo-tag for masking the toxicity to the host and providing cleavage site to remove the tags attached to the peptide, (3) triple peptide tandem with the GGGGS linkers. We used the T7 promoter to control the peptide expression. The ribosomal binding site and T7 terminator were added for full protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 545
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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