Part:BBa_K3798010
ssDNA, an aptamer for Ag+ ion
Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K37980010 is a Ag+ aptamer obtained from a previous paper[1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
Binding efficiency and stability
Aptamer 9 shows a binding efficacy of only 36%, and was not stable in performance. Therefore, this aptamer is discarded and was not subjected for further examination.
Reference
1. Saran, R., Yao, L., Hoang, P., & Liu, J. (2018). Folding of the silver aptamer in a DNAzyme probed by 2-aminopurine fluorescence. Biochimie, 145, 145-150. doi: 10.1016/j.biochi.2017.07.001
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