Part:BBa_M10018

{˂Z34C domain˃} This part is a bglbricks basic part for the coding sequence of a synthetic Protein A. Protein A is a protein found in Staphylococcus aureus that is good at binding to IgG antibodies. This part is the engineered Protein A domain found in: PMID: 9294166. This part binds very well to only the constant region (Fc) of IgG.

Background: Protein A is a protein found in Staphylococcus aureus that is good at binding to IgG antibodies. There's two sites on IgG that it can bind to. The fab and the fc portions. The fab is of the the 2 heads of the y antibody and the fc is the (constant region) tail. Since only the Fc portion is interesting, an effort was made to find the smallest protein domain possible that still binds to the fc portion of IgG. Phage display was used to select for smaller proteins which still bind to IgG which resulted in the development of Z34C which binds to fc, but loses the ability to bind to fab. PMID: 9294166

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This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]