Part:BBa_M10025

{˂GFP-LVA˃} Fast-Degrading GFP

The purpose of this part is to encode a fast degrading GFP. Wildtype GFP typically have a half-life that may last for several bacterial generations. However, the addition of an AANDENYALAA tag to the C-terminal of the wildtype GFP will decrease this halflife to 40 minutes (Andersen et al, 1998). By changing the last three residues of this tag, various fast-degrading GFP were created. GFP[LVA] has a halflife of 40 minutes (Miller et al., 2000).

Usage of a fast degrading GFP may act as a better reporter of changes in gene transcription over a period of time.

PMID: 11059491

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This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 716
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 716
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 716
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 716
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 641