Part:BBa_K3798005

ssDNA, our best aptamer for Ag+ ion 2

Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K3798005 is a Ag+ aptamer obtained from a previous paper[1], and this particular aptamer had shown outstanding abilities efficiency and satbility in Ag+ ion capture.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Binding efficiency and stability

Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers.

As can see above aptamer 3 and 4 has the best binding efficiency with Aptamer 3 having a higher binding ability (the remaining ion concentration of Aptamer 3 is 86ug/L lower than that of Aptamer 4) and Aptamer 4 having better stability when binding (smaller error bounds). Because having 90% of binding efficiency is enough for our use and having higher binding stability means that the treated effluents would have a more consistent results that is essential for industrial use, Aptamer 4 was chosen to be subject of further experiments.

Reference

1. Shen, F., Mao, S., Mathivanan, J., Wu, Y., Chandrasekaran, A., & Liu, H. et al. (2020). Short DNA Oligonucleotide as a Ag+ Binding Detector. ACS Omega, 5(44), 28565-28570. doi: 10.1021/acsomega.0c03372