Part:BBa_M10024:Design

{a~Int-native>} Intimin (native)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 405
  Illegal PstI site found at 653
  Illegal PstI site found at 1100
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1248
  

Design Notes

Construction of {a~Inv-native>} basic part PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (146 bp, gp = A) PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1889 bp, gp = B)

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PCR Bhs001F/Bhs003R on A+B (2009 bp, EcoRI/BamHI) Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10024 {a~Inv-native>}

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Bhs001F Forward EcoRI for {a~Inv-native>} cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG Bhs002F Removing the EcoRI site from {a~Inv-native>} GTTAATCAGAACTCATTTGCAAATGG Bhs002R Removing the EcoRI site from {a~Inv-native>} CCATTTGCAAATGAGTTCTGATTAAC Bhs003R Reverse BamHI for {a~Inv-native>} GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC

Source

Genomic sequence of E. coli strain 0157:H7