Part:BBa_M10025
{˂GFP-LVA˃} Fast-Degrading GFP
The purpose of this part is to encode a fast degrading GFP. Wildtype GFP typically have a half-life that may last for several bacterial generations. However, the addition of an AANDENYALAA tag to the C-terminal of the wildtype GFP will decrease this halflife to 40 minutes (Andersen et al, 1998). By changing the last three residues of this tag, various fast-degrading GFP were created. GFP[LVA] has a halflife of 40 minutes (Miller et al., 2000).
Usage of a fast degrading GFP may act as a better reporter of changes in gene transcription over a period of time.
This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 716
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 716
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 716
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 716
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
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