Tag
FLAG

Part:BBa_M10019:Design

Designed by: John Wang   Group: Berkeley BioE140L - S09   (2009-05-09)
Revision as of 01:03, 10 May 2009 by Registry (Talk | contribs)

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{˂FLAG˃}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Later


Source

Wobble PCR Jw0019F / Jw0020R (45 bp, EcoRI/BamHI)

Sub into pBca1256 (EcoRI/BamHI, 2472+ 910, L)

Product is pBca1256-K112602 {Flag}

Jw0019F Forward construction of Flag

cgataGAATTCatgAGATCTgactacaaggatgacgacg

Jw0020R Reverse construction of Flag

ccagtGGATCCcttgtcgtcgtcatccttgtagtc


References