Coding

Part:BBa_M10025:Design

Designed by: Hank Shih   Group: Berkeley BioE140L - S09   (2009-05-09)
Revision as of 00:55, 10 May 2009 by Hshih21 (Talk | contribs) (→‎Source)

{˂GFP-LVA˃} Fast-Degrading GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


Design Notes

This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]


This part was made using a LR Gateway Transfer reaction. More information on this process can be found here:
[http://2008.igem.org/Team:UC_Berkeley/GatewayOverview LR Gateway Transfer Overview]

Source

pBca1256-Bjh1857

References