Composite

Part:BBa_K3926006

Designed by: Zhipeng Gao   Group: iGEM21_XHD-Wuhan-A-China   (2021-10-19)
Revision as of 11:35, 19 October 2021 by Registry (Talk | contribs)

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amilCP report napA expression

uses a strong promoter (BBa_J23100), strong RBS (BBa_B0034), napA coding region (BBa_K3926004), and a double terminator (BBa_B0015). Will generate amilCP when napA expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 2415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1263
    Illegal BglII site found at 1407
    Illegal XhoI site found at 1130
    Illegal XhoI site found at 2240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 107
    Illegal NgoMIV site found at 1357
    Illegal NgoMIV site found at 1645
    Illegal NgoMIV site found at 1801
    Illegal NgoMIV site found at 2185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1594
    Illegal BsaI.rc site found at 1553
    Illegal SapI site found at 1858


[edit]
Categories
Parameters
None