Composite

Part:BBa_K3941005

Designed by: Can Bora Ciner   Group: iGEM21_Saint_Joseph   (2021-10-17)
Revision as of 11:10, 19 October 2021 by Boraciner (Talk | contribs)

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pET29b+EGII

BBa_K3941005 is a composite part that we used in our expression plasmids (pET29b). We obtain EG2 wild type with this sequence.

This composite part contains 4 parts:

- T7 Promoter (BBa_K3941000)
- LacO (BBa_K1624002)
- EGII (BBa_K3941001)
- T7 Terminator


800px-T--Saint_Joseph--EGII-Whole.png

Figure 1: T7 promoter, Lac Operon, Codon optimized EGII, 6XHis and terminator


Codon Optimized EGII

This composite part originated from a codon-optimized (for E. coli DH5⍺) version of the endoglucanase (EG) gene that cleaves the internal beta-1,4-glycosidic bonds in cellulose. We optimized the sequence for expression and added a 6XHis at the end.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 22
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None