Part:BBa_K3994009

PttB344_HrpS_PJ23105_ttrR

promoter

Usage and Biology

Background

Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease of unknown etiology, including ulcerative colitis (CD) and Crohn’s disease (CD). This chronic disease, which is prone to repeated deterioration, currently lacks unified diagnostic and treatment standards, and is posing a great threat to public health. Drug therapy (anti-inflammatory drugs) is the preferred treatment for IBD. However, studies in the past 10 years have found that 30-50% of IBD patients do not respond to anti-TNF treatment. In addition, after long-term use of anti-inflammatory drugs, the patient's intestinal microbial status changes over time, and the effect may be lost due to drug resistance. Therefore, we need to seek help from other treatments for IBD. HrpR protein binds to HrpS protein forming a complex and then triggering the transcription of promoter hrpL. It can sense S4O62- to release the substance S

Construct design

HrpS is a key functional factor under PttB344 promoter (Figure 1). And this sequence is inserted into plasmid. Its sequence is shown in Figure 2.

Figure 2a. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 25
  Illegal XbaI site found at 175
  Illegal PstI site found at 13
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 839
  Illegal NheI site found at 862
  Illegal PstI site found at 13
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1893
  Illegal BamHI site found at 31
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 25
  Illegal XbaI site found at 175
  Illegal PstI site found at 13
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 25
  Illegal XbaI site found at 175
  Illegal PstI site found at 13
  Illegal AgeI site found at 553
  Illegal AgeI site found at 1106
  Illegal AgeI site found at 1112
  Illegal AgeI site found at 1247
  
• 1000
  COMPATIBLE WITH RFC[1000]