Composite

Part:BBa_K4032104

Designed by: Tsugumi Ohzeki   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 09:20, 19 October 2021 by Tsugumi (Talk | contribs)


lacI+lacZ+RBS+amylase-RFP+double terminator

This part contains sequences of fuse protein of amylase and RFP.

This part is the addition of a lac promoter and double terminator to BBa_K40320xx. Lac promoter and double terminator were used as contained in BBa_J04450.

This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. Red fluorescence of RFP is observed under the fluorescence microscope.

This part was created by In-Fusion method using BBa_J04450 as inverse and BBa_K523006 as insert.

plasmid image



Fig. 1 method


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 977
    Illegal AgeI site found at 2142
    Illegal AgeI site found at 3221
    Illegal AgeI site found at 3333
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None