Composite

Part:BBa_K3823009

Designed by: Yiying Zhang   Group: iGEM21_Tongji_China   (2021-10-01)
Revision as of 08:39, 19 October 2021 by Hanqing (Talk | contribs)


SQR-SDO-AprBA-SAT

Sulfide: quinone oxidoreductase(SQR), is an ancient flavoprotein of the disulfide oxidoreductase family that is present in nearly all domains of life. SQRs were first found in sulfide trophic bacteria, later SQR-like enzymes were found in the mitochondria of some fungi, as well as in all animal species whose genomes have been sequenced . Several SQRs have been purified and characterized by biochemical methods. They are considered to be integral monotopic membrane proteins, associating with the membrane through amphipathic helices. The monomeric molecular mass of the enzyme is around 50 kDa. The enzyme usually harbors a covalently-bound FAD cofactor in each monomer. However, FAD can also be non-covalently bound as in the SQR of A. ferrooxidans and some other organisms.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3324
    Illegal SpeI site found at 1797
    Illegal PstI site found at 1514
    Illegal PstI site found at 2726
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1465
    Illegal NheI site found at 1488
    Illegal NheI site found at 2311
    Illegal NheI site found at 2334
    Illegal NheI site found at 3216
    Illegal SpeI site found at 1797
    Illegal PstI site found at 1514
    Illegal PstI site found at 2726
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1997
    Illegal BamHI site found at 1373
    Illegal BamHI site found at 3529
    Illegal BamHI site found at 4984
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3324
    Illegal SpeI site found at 1797
    Illegal PstI site found at 1514
    Illegal PstI site found at 2726
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3324
    Illegal SpeI site found at 1797
    Illegal PstI site found at 1514
    Illegal PstI site found at 2726
    Illegal NgoMIV site found at 109
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 1723
    Illegal NgoMIV site found at 4003
    Illegal NgoMIV site found at 4174
    Illegal NgoMIV site found at 4857
    Illegal AgeI site found at 1028
    Illegal AgeI site found at 1744
    Illegal AgeI site found at 2537
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None