Coding

Part:BBa_K3991000

Designed by: Ke ZHU   Group: iGEM21_Shanghai_United   (2021-10-12)
Revision as of 07:37, 19 October 2021 by Hsd1202 (Talk | contribs)


ArsD

Profile

Name: ArsD

Base Pairs: 1200 bp

Origin: Escherichia coli (strain: LST424C, nat-host: Homo sapiens)

Properties: Gene technology for protecting patented bacterial strains

ArsD is a trans-acting repressor of the arsRDABC operon that confers resistance to arsenicals and antimonials in Escherichia coli.

Experimental approach

Production, purification, and sequcing analysis of recombinant ArsD-amilGFP

Figure 1: Gel Electrophoresis Results of PCR of amilGFP Genes..

Figure 1 shows gel electrophoresis results of amilGFP PCR. Column M is a 2K marker ladder. Columns 1-6 are PCR products of amilGFP genes All 1-6 columns displayed successful results at 700bp which could be used for later experiments. 



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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